Producing a superior genotype from agria potato cultivar using somaclonal variation

Authors

  • Eshaghali Bayati Department of Agronomy and Plant Breeding, Arak Branch, Islamic Azad University, Arak, Iran
  • Masoud Gomarian Department of Agronomy and Plant Breeding, Arak Branch, Islamic Azad University, Arak, Iran
  • Hossein Mirzaie-Nodousha Biotechnology Department, Research Institute of Forests and Rangelands, Tehran, Iran
  • Mehdi Changizi Department of Agronomy and Plant Breeding, Arak Branch, Islamic Azad University, Arak, Iran
  • Shahab Khaghani Department of Agronomy and Plant Breeding, Arak Branch, Islamic Azad University, Arak, Iran

DOI:

https://doi.org/10.5377/nexo.v34i02.11551

Keywords:

CBDP marker, morphological traits, potato, somaclonal variation

Abstract

This study was performed to produce a superior genotype from Agria potato cultivar using somaclonal variation. Two leaf and meristem explants in combination with four doses of 2,4-D (0, 2, 3 and 4 mg / l) were used for callus induction in a factorial model based on a completely randomized design with 3 replications. Results showed that the meristem explant, along with 3 mg 2,4-D produced the most suitable calluses. In the mentioned regeneration media, the best calluses were regenerated and one of the regenerated genotypes, which was very different from the parent cultivar was selected. The regenerated genotype, was compared with the maternal genotype (Agria) and a control cultivar (Sante), in a field experiment based on a randomized complete block design with 3 replications. The results showed that in terms of most of the studied traits such as tuber weight per plant, stolon length, percentage of dry matter and percentage of starch, the new genotype was superior, compared to the parent cultivar and in terms of peel percentage and maturity date, the parental cultivar was superior. The results of the molecular comparison also showed that based on CBDP marker, both in terms of band number and band size, there were differences between the new genotype and the parental cultivar. In general, results showed that somaclonal variation can be an effective method to generate new genotypes with superior characteristics.

Downloads

Download data is not yet available.

Downloads

Published

2021-06-07

How to Cite

Bayati, E., Gomarian, M., Mirzaie-Nodousha, H., Changizi, M., & Khaghani, S. (2021). Producing a superior genotype from agria potato cultivar using somaclonal variation. Nexo Scientific Journal, 34(02), 671–681. https://doi.org/10.5377/nexo.v34i02.11551

Issue

Vol. 34 No. 02 (2021)

Section

Articles

License

The authors who publish in Nexo Scientific Journal agree to the following terms:

  • Authors retain the copyright and grant the journal the right of the first publication under the license Creative Commons Attribution License https://creativecommons.org/licenses/by/3.0/, which allows others to share the work with a recognition of the authorship of the work and the initial publication in Nexo Scientific Journal.
  • Authors may separately establish additional agreements for the non-exclusive distribution of the version of the work published in the journal (for example, in an institutional repository or a book), with the recognition of the initial publication in Nexo Scientific Journal.
  • Authors are allowed and encouraged to disseminate their works electronically (for example, in institutional repositories or in their own website) before and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published works.